Nível
Mestrado/Doutorado
Natureza
Semestral
Créditos
03
Carga horária
45 horas
Ementa
Protocolos para fixação, histoquímica, inclusão e microtomia para análise em microscopia de luz.
Semestre
1º
Dia/Horário
A definir
Docente
Bruno Francisco Sant’Anna dos Santos
Bibliografia Recomendada
ATKINSON, J.A.; WELLS, D.M. An updated protocol for high throughput plant tissue sectioning. Frontiers in plant science, 2017, 8: 1721.
BELLINGER, M.A.; SIDHU, S.K.; RASMUSSEN, C.G. Staining Maize Epidermal Leaf Peels with Toluidine Blue O. Bio-101: e3214, 2019, DOI: 10.21769/BioProtoc.3214.
CLODE, P.L. A Method for Preparing Difficult Plant Tissues for Light and Electron Microscopy. Microscopy and Microanalysis, 2015, 21: 902-909.
CRANG, R.; LYONS-SOBASKI, S.; WISE, R. Microscopy and Imaging. In: Plant Anatomy. Springer, Cham, 2018. p. 45-74.
VIDAL, B.C..; MELLO, M.L.S. Toluidine blue staining for cell and tissue biology applications. Acta histochemica, 2019, 121: 101-112.
DEMARCO, D. Histochemical analysis of plant secretory structures. In: Histochemistry of Single Molecules. Humana Press, New York, NY, 2017. p. 313-330.
DOUGLAS, S.M. Cytology, histology, and histochemistry of MLO infections in tree fruits. Cytology, Histology and Histochemistry of Fruit Tree Diseases, 2019, 23.
EVERT, R.F. Esau’s plant anatomy: meristems, cells, and tissues of the plant body: their structure, function, and development. 3., 2015.
LUX, A.; VACULÍK, M.; KOVÁČ, J. Improved methods for clearing and staining of plant samples. In: Plant microtechniques and protocols. Springer, Cham, 2015. p. 167-178.
OLIVEIRA, J.M.S. Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues. Acta Botanica Brasilica, 2015, 29.2: 207-212.
ROCHA, D. I., KURCZYŃSKA, E., POTOCKA, I., STEINMACHER, D. A., OTONI, W. C. Histology and histochemistry of somatic embryogenesis. In Somatic Embryogenesis: Fundamental Aspects and Applications (pp. 471-494). Springer, Cham, 2016.
TIMMERS, A.C.J. Light microscopy of whole plant organs. Journal of microscopy, 2016, 263.2: 165-170.
VERHERTBRUGGEN, Y., WALKER, J.L., GUILLON, F., AND SCHELLER, H.V. A Comparative Study of Sample Preparation for Staining and Immunodetection of Plant Cell Walls by Light Microscopy. United States: N. p., 2017. Web. doi:10.3389/fpls.2017.01505.
