{"id":901,"date":"2018-08-22T19:15:25","date_gmt":"2018-08-22T19:15:25","guid":{"rendered":"https:\/\/agrarias.ufpr.br\/pgapv\/?page_id=901"},"modified":"2026-02-04T20:16:46","modified_gmt":"2026-02-04T20:16:46","slug":"agro-xxxx-processamento-de-amostras-para-estudos-em-anatomia-vegetal","status":"publish","type":"page","link":"https:\/\/agrarias.ufpr.br\/pgapven\/agro-xxxx-processamento-de-amostras-para-estudos-em-anatomia-vegetal\/","title":{"rendered":"AGRO 7040 – Sample Processing For Plant Anatomy Studies"},"content":{"rendered":"\n

 <\/strong>Level<\/strong><\/span><\/span><\/p>\n\n\n\n

Master\/Doctoral<\/span><\/p>\n\n\n\n

Credits <\/strong><\/span><\/p>\n\n\n\n

03 (45 h)<\/span><\/p>\n\n\n\n

Content: <\/strong><\/span><\/p>\n\n\n\n

Protocols for fixation, histochemistry, inclusion and microtomy for under light microscopy.<\/span><\/p>\n\n\n\n

Professor<\/strong><\/span><\/p>\n\n\n\n

Bruno Francisco Sant\u2019Anna dos Santos<\/a><\/span><\/p>\n\n\n\n

References<\/b><\/span><\/p>\n\n\n\n

ATKINSON, J.A.; WELLS, D.M. An updated protocol for high throughput plant tissue sectioning. Frontiers in plant science, 2017, 8: 1721.<\/span><\/p>\n\n\n\n

BELLINGER, M.A.; SIDHU, S.K.; RASMUSSEN, C.G. Staining Maize Epidermal Leaf Peels with Toluidine Blue O. Bio-101: e3214, 2019, DOI: 10.21769\/BioProtoc.3214.<\/span><\/p>\n\n\n\n

CLODE, P.L. A Method for Preparing Difficult Plant Tissues for Light and Electron Microscopy. Microscopy and Microanalysis, 2015, 21: 902-909.<\/span><\/p>\n\n\n\n

CRANG, R.; LYONS-SOBASKI, S.; WISE, R. Microscopy and Imaging. In: Plant Anatomy. Springer, Cham, 2018. p. 45-74.<\/span><\/p>\n\n\n\n

VIDAL, B.C..; MELLO, M.L.S. Toluidine blue staining for cell and tissue biology applications. Acta histochemica, 2019, 121: 101-112.<\/span><\/p>\n\n\n\n

DEMARCO, D. Histochemical analysis of plant secretory structures. In: Histochemistry of Single Molecules. Humana Press, New York, NY, 2017. p. 313-330.<\/span><\/p>\n\n\n\n

DOUGLAS, S.M. Cytology, histology, and histochemistry of MLO infections in tree fruits. Cytology, Histology and Histochemistry of Fruit Tree Diseases, 2019, 23.<\/span><\/p>\n\n\n\n

EVERT, R.F. Esau\u2019s plant anatomy: meristems, cells, and tissues of the plant body: their structure, function, and development. 3., 2015.<\/span><\/p>\n\n\n\n

LUX, A.; VACUL\u00cdK, M.; KOV\u00c1\u010c, J. Improved methods for clearing and staining of plant samples. In: Plant microtechniques and protocols. Springer, Cham, 2015. p. 167-178.<\/span><\/p>\n\n\n\n

OLIVEIRA, J.M.S. Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues. Acta Botanica Brasilica, 2015, 29.2: 207-212.<\/span><\/p>\n\n\n\n

ROCHA, D. I., KURCZY\u0143SKA, E., POTOCKA, I., STEINMACHER, D. A., OTONI, W. C. Histology and histochemistry of somatic embryogenesis. In Somatic Embryogenesis: Fundamental Aspects and Applications (pp. 471-494). Springer, Cham, 2016.<\/span><\/p>\n\n\n\n

TIMMERS, A.C.J. Light microscopy of whole plant organs. Journal of microscopy, 2016, 263.2: 165-170.<\/span><\/p>\n\n\n\n

VERHERTBRUGGEN, Y., WALKER, J.L., GUILLON, F., AND SCHELLER, H.V. A Comparative Study of Sample Preparation for Staining and Immunodetection of Plant Cell Walls by Light Microscopy. United States: N. p., 2017. Web. doi:10.3389\/fpls.2017.01505.<\/span><\/p>\n\n\n\n

 <\/strong> <\/span><\/span><\/p>\n\n\n\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

 Level Master\/Doctoral Credits 03 (45 h) Content: Protocols for fixation, histochemistry, inclusion and microtomy for under light microscopy. Professor Bruno Francisco Sant\u2019Anna dos Santos References ATKINSON, J.A.; WELLS, D.M. An updated protocol for high throughput plant tissue sectioning. Frontiers in plant science, 2017, 8: 1721. BELLINGER, M.A.; SIDHU, S.K.; RASMUSSEN, C.G. Staining Maize Epidermal Leaf […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-901","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/901","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/comments?post=901"}],"version-history":[{"count":10,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/901\/revisions"}],"predecessor-version":[{"id":32340,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/901\/revisions\/32340"}],"wp:attachment":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/media?parent=901"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}