{"id":4370,"date":"2021-03-03T17:07:23","date_gmt":"2021-03-03T17:07:23","guid":{"rendered":"https:\/\/agrarias.ufpr.br\/pgapven\/?page_id=4370"},"modified":"2026-02-04T20:14:25","modified_gmt":"2026-02-04T20:14:25","slug":"agro-7044-molecular-detection-of-phytopathogens","status":"publish","type":"page","link":"https:\/\/agrarias.ufpr.br\/pgapven\/agro-7044-molecular-detection-of-phytopathogens\/","title":{"rendered":"AGRO 7044 – Molecular Detection of Phytopathogens"},"content":{"rendered":"\n

Level<\/strong><\/span><\/p>\n\n\n\n

Master\/Doctoral<\/span><\/p>\n\n\n\n

Credits<\/strong><\/span><\/p>\n\n\n\n

04 (60 h)<\/span><\/p>\n\n\n\n

Content:<\/strong><\/span><\/p>\n\n\n\n

Studies on molecular techniques used for detection and identification of plant diseases.  Information on DNA sequencing and basic bioinformatics for data analysis. Studies on Molecular Markers applied to phytopathology and on serological techniques for identifying pathogens.<\/span><\/p>\n\n\n\n

Professor<\/b><\/span><\/p>\n\n\n\n

Renata Faier Calegario<\/a><\/span><\/p>\n\n\n\n

References<\/strong><\/span><\/p>\n\n\n\n

BRASILEIRO, A.C.M., CARNEIRO, V.T.de C. Manual de transforma\u00e7\u00e3o gen\u00e9tica de plantas. 2\u00aa ed. Bras\u00edlia: EMBRAPA, 2015. p.456<\/span><\/p>\n\n\n\n

EIRAS, M.; GALLETI S.R. (Editores t\u00e9cnicos). T\u00e9cnicas de diagn\u00f3stico de fitopat\u00f3genos. S\u00e3o Paulo: Devir, 2012.<\/span><\/p>\n\n\n\n

HAMPTOM, R., BALL, E. & DE BOER, S. (Eds.). Serological methods for detection and identification of viral and bacterial plant pathogens \u2013 A Laboratory Manual. APS Press, St.<\/span><\/p>\n\n\n\n

JANSSEN, T., KARSSEN, G., COUVREUR, M., WAEYENBERGE, L., BERT, W. The pitfalls of molecular species identification: a case study within the genus Pratylenchus (Nematoda:<\/span><\/p>\n\n\n\n

Koressaar T, Lepamets M, Kaplinski L, Raime K, Andreson R and Remm M. Primer3_masker: integrating masking of template sequence with primer design software.<\/span><\/p>\n\n\n\n

MALAJOVICH M. A. Biotecnologia 2011. Rio de Janeiro, Edi\u00e7\u00f5es da Biblioteca Max Feffer do Instituto de Tecnologia ORT, 2012.<\/span><\/p>\n\n\n\n

Malarczyk, D., Panek, J., Frac, M. Alternative Molecular-Based Diagnostic Methods of Plant Pathogenic Fungi Affecting Berry Crops-A Review. Molecules Molecules 2019, 24,<\/span><\/p>\n\n\n\n

Martinelli, f.,   Scalenghe, r., Davino, s Panno, s., Scuderi, g., Ruisi, p., Villa, p., Stroppiana, d., Boschetti, m., Goulart, l.r., Davis, c.e., Dandekar, a.m. Advanced methods of plant<\/span><\/p>\n\n\n\n

MORGANTE, C.V.; BLAWID, R. An\u00e1lise da express\u00e3o g\u00eanica pela t\u00e9cnica de PCR quantitativa em tempo real: princ\u00edpios e fundamentos. Petrolina: Embrapa Semi\u00e1rido, Documentos, 278, 2016. 63 p. <\/span><\/p>\n\n\n\n

Nadeem, M.A., Nawaz, M.A., Shahid, M.Q., Do\u011fan, Y., Comertpay, G., Y\u0131ld\u0131z, M., Hatipo\u011flu, R., Ahmad, F.,  Alsaleh, A., Labhane, N., \u00d6zkan, H., Chung G., Baloch, F.S. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing, Biotechnology & Biotechnological Equipment, 2018, 32:2, 261-285.<\/span><\/p>\n\n\n\n

OLIVEIRA, E.M.M; de Oliveira, t.C.; de Souza, A.M.; dos Santos, T.F.; de Lima, I.S. Desenho de Primers Degenerados atrav\u00e9s de Bioinform\u00e1tica. Rio de Janeiro: Embrapa<\/span><\/p>\n\n\n\n

QUISEN, R.C.; \u00c2NGELO, P.C.S. Manual de procedimentos do Laborat\u00f3rio de Cultura de Tecidos da Embrapa Amaz\u00f4nia Ocidental. Manaus: Embrapa Amaz\u00f4nia Ocidental. Documentos 61. 2008. 44p.<\/span><\/p>\n\n\n\n

ROMANO, E.; BRASILEIRO, A.C.M. Extra\u00e7\u00e3o de DNA de plantas. Biotecnologia, Ci\u00eancia e Desenvolvimento, v. 2, n. 9, p. 40-43, 1999.<\/span><\/p>\n\n\n\n

RUBIO, L.; GALIPIENSO, L.; FERRIOL, I. Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution. Front. Plant Sci., 11, 2020. <\/span><\/p>\n\n\n\n

ZAHA, A., FERREIRA, H.B., PASSAGLIA, L.M.P. Biologia molecular b\u00e1sica [recurso eletr\u00f4nico]. 5. ed. Dados eletr\u00f4nicos. Porto Alegre: Artmed, 2014.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Level Master\/Doctoral Credits 04 (60 h) Content: Studies on molecular techniques used for detection and identification of plant diseases.  Information on DNA sequencing and basic bioinformatics for data analysis. Studies on Molecular Markers applied to phytopathology and on serological techniques for identifying pathogens. Professor Renata Faier Calegario References BRASILEIRO, A.C.M., CARNEIRO, V.T.de C. Manual de […]<\/p>\n","protected":false},"author":10271,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4370","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/4370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/users\/10271"}],"replies":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/comments?post=4370"}],"version-history":[{"count":5,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/4370\/revisions"}],"predecessor-version":[{"id":32323,"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/pages\/4370\/revisions\/32323"}],"wp:attachment":[{"href":"https:\/\/agrarias.ufpr.br\/pgapven\/wp-json\/wp\/v2\/media?parent=4370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}