Level

Master/Doctoral

Credits

04 (60 h)

Content

This course aims at broadening knowledges about plant tissue culture techniques used for breeding and improvement of cultivated species. The main techniques studied are: cell and protoplast cultures, somatic hybridization, genetic transformation, genome edition and haploid production. The use of thesemethods for plant improvement is explained as well as their advantages and disadvantages, and pratical cases are discussed.

Professor

Marguerite Quoirin

References

ALVAREZ, M. A.(edit.) Genetic Transformation. InTech Publisher, 328 p.,

ANIS, M. e NASEEM, A. Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, 2016. DOI 10.1007/978-981-10-1917-

BORÉM, A. Biotecnologia Florestal. Viçosa, MG: Universidade Federal de Viçosa, 2007

CHADHA, K.L.; RAVINDRAN, P.N. e SAHIJRAM, L. Biotechnology in horticultural and plantation crops, 2000. http://bpagency.com

CHEN, K.; WANG, Y.; ZHANG, H.; GAO, C. 2019. CRISP/Cas genome editing and precision plant breeding in agriculture. Annual Review of Plant Biology, v. 70 (1), p. 667-697.

DIXON, R. A. e GONZALES, R. A. Plant Cell Culture. A Practical Approach. 2nd ed. The Practical Approach Series. RICKWOOD, D. & HAMES, B.D. edit. IRL Press, Oxford University Press, UK, 2003.

GEORGE, E. F. Plant propagation by tissue culture: the technology. Great Britain: Exegetics Limited, vol. 1, 1993; vol. 2, 1996.

GEORGE, E. F.; HALL, M. A. e DE KLERK, G.-J. Plant Propagation by Tissue Culture. Vol. I. The Background. 3d ed. Dordrecht: Springer, 2008.

GUPTA, S. D. e IBARAKI, Y. (edit.). Plant Tissue Culture Engineering. Springer. Focus on Biotechnology, 2008, 480 p. DOI 10.1007/978-1-4020-3694-1.

JAIN, S.M. e GUPTA, P.K. Protocol for Somatic Embryogenesis in Woody Plants. Dordrecht: Springer, 2005.

MURCH, S.J. e SAXENA, P.K. Journey of a Single Cell to a Plant. Plymouth, UK: Science Publishers, 2005. TORRES, A. C.; CALDAS, L.S. e BUSO, J. A. Cultura de tecidos e transformação genética de plantas. Brasília:,EMBRAPA – CBAB, vol.1, 1998 e vol. 2, 1999.

TORRES, A.C.; DUSI, A. N. e SANTOS, M.D.M. dos. Transformação Genética de Plantas via Agrobacterium. Brasília: EMBRAPA Hortaliças, 2007.