Level
Master/Doctoral
Credits
04 (60 h)
Content:
Morphological and physiological bases of in vitro culture. Micropropagation techniques (organogenesis: multiplication of axillary shoots, meristem and shoot
culture, regeneration of adventitious shoots, and somatic embryogenesis): stages, advantages, disadvantages and applications. Problems in the induction and
maintenance of in vitro cultures.
Professor
References
ALMEIDA, M. et al. 2015. Plant morphogenesis: theorical bases. Advances in Forest Science, v.2, n.1, p.13-22.
BAIRU, M. & KANE, M.E. 2011. Physiological and developmental problems encountered by in vitro culture plants. Plant Growth Regulation, 63: 101-103.
ELMEER, K.E.S. 2013. Factors regulating somatic embryogenesis in plants. In: ASLAM, J.; SRIVASTAVA, P.S.; SHARMA, M. P. Somatic embryogenesis and gene expression. New Dheli: Narosa Publishing. p. 56-81.
FEHÉR, A. 2015. Somatic embryogenesis? stress induced remodeling of plant cell fate. Biochimica et Biophysica Acta, v.1849, p. 385-402.
GEORGE, E. F.; HALL, M. A.; DE KLERK, G.J. 2008. Plant propagation by tissue culture 3rd edition. Dordrecht: Springer.
GUAN, Y.; LI, S.G.; FAN, X.F.; SU, Z.H. 2016. Applications of somatic embryogenesis in woody plants. Frontiers in Plant Science, 7:1-12.
ISAH, T. Adjustments to in vitro culture conditions and associated anomalies in plants. Acta Biologica Cracoviensia, v. 57, n.2, p.9-28, 2015.
KUMAR, K. & RAO, I.U. 2012. Morphophysiologicals problems in acclimatization of micropropagated plants in ex vitro conditions ? a reviews. Journal Ornamental and Horticultural Plants. 2:271-283.
MÉNDEZ-HERNÁNDEZ, H. A. et al. Signaling overview of plant somatic embryogenesis. Frontiers in Plant Science, v. 10. p. 1-15, 2019.
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PHILLIPS, G. C.; GARDA, M. Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology Plant. v. 55, p.242-257, 2019.
REDDY, M.C.; MURTHY, K. S. R.; PULLAIAH, T. 2012. Synthetic seeds: a review in agriculture and forestry. African Journal of Biotechnology, 11: 14254-14275.
SHIN, J.; BAE, S.; SEO, P. J. De novo shoot organogenesis during plant regeneration. Journal of Experimental Botany, v. 71, n.1, p. 63-72, 2020.
RUFFONI, B. & SAVONA, M. 2013. Physiological and biochemical analysis of growth abnormalities associated with plant tissue culture. Horticulture Environment and. Biotechnology, 54: 191-205.
SING, C. R. Review on problems and its remedy in plant tissue culture. Asian Journal of Biological Sciences, p. 1-8, 2018.
SOH, W.Y & BHOJWANI, S.S. 2010. Morphogenesis in plant tissue cultures. Netherlands: Kluwer Academic.
TEIXEIRA DA SILVA, J. A.; DOBRÁNSZKI, J. Recent advances and novelties in the thin cell layer-based plant biotechnology – a mini review. BioTechnologia, v. 100, p. 89-96, 2019.
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