{"id":3280,"date":"2021-02-15T01:38:23","date_gmt":"2021-02-15T01:38:23","guid":{"rendered":"https:\/\/agrarias.ufpr.br\/pgapv\/?page_id=3280"},"modified":"2025-04-24T16:36:57","modified_gmt":"2025-04-24T16:36:57","slug":"agro-7044-deteccao-molecular-de-fitopatogenos","status":"publish","type":"page","link":"https:\/\/agrarias.ufpr.br\/pgapv\/agro-7044-deteccao-molecular-de-fitopatogenos\/","title":{"rendered":"AGRO 7044 – Detec\u00e7\u00e3o Molecular de Fitopat\u00f3genos"},"content":{"rendered":"

N\u00edvel<\/strong><\/p>\n

Mestrado\/Doutorado<\/p>\n

Natureza<\/strong><\/p>\n

Concentrada<\/p>\n

Cr\u00e9ditos<\/strong><\/p>\n

04<\/p>\n

Carga hor\u00e1ria<\/strong><\/p>\n

60 horas<\/p>\n

Ementa<\/strong><\/p>\n

Estudos de t\u00e9cnicas moleculares utilizadas para detec\u00e7\u00e3o e identifica\u00e7\u00e3o de fitopat\u00f3genos. Informa\u00e7\u00f5es sobre extra\u00e7\u00e3o de \u00e1cidos nucleicos, PCR e suas varia\u00e7\u00f5es, qPCR, sequenciamento de Sanger, bioinform\u00e1tica b\u00e1sica para an\u00e1lise de sequ\u00eancias e elabora\u00e7\u00e3o de \u00e1rvores filogen\u00e9ticas. Estudos sobre t\u00e9cnicas sorol\u00f3gicas para identifica\u00e7\u00e3o de fitopat\u00f3genos.<\/p>\n

Semestre<\/strong><\/p>\n

2\u00ba<\/p>\n

Dia\/Hor\u00e1rio<\/strong><\/p>\n

A definir<\/p>\n

Docente<\/strong><\/p>\n

Renata Faier Calegario<\/a><\/p>\n

Bibliografia Recomendada<\/strong><\/p>\n

BRASILEIRO, A.C.M.; CARNEIRO, V.T. de C. Manual de transforma\u00e7\u00e3o gen\u00e9tica de plantas<\/em>. 2. ed. Bras\u00edlia: EMBRAPA, 2015. p. 456.<\/p>\n

EIRAS, M.; GALLETI, S.R. (Eds.). T\u00e9cnicas de diagn\u00f3stico de fitopat\u00f3genos<\/em>. S\u00e3o Paulo: Devir, 2012.<\/p>\n

GREEN, Michael R.; SAMBROOK, Joseph. Molecular Cloning: A Laboratory Manual<\/em>. 4. ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2012. 3 v.\u200b<\/p>\n

HAMPTON, R.; BALL, E.; DE BOER, S. (Eds.). Serological methods for detection and identification of viral and bacterial plant pathogens \u2013 A Laboratory Manual<\/em>. St. Paul: APS Press, 1990.<\/p>\n

JANSSEN, T.; KARSSEN, G.; COUVREUR, M.; WAEYENBERGE, L.; BERT, W. The pitfalls of molecular species identification: a case study within the genus Pratylenchus<\/em> (Nematoda: Pratylenchidae). Nematology<\/em>, v. 19, n. 10, p. 1179-1199, 2017.<\/p>\n

KORESSAAR, T.; LEPAMETS, M.; KAPLINSKI, L.; RAIME, K.; ANDRESON, R.; REMM, M. Primer3_masker: integrating masking of template sequence with primer design software. Bioinformatics<\/em>, v. 30, n. 4, p. 548-550, 2014.<\/p>\n

MALAJOVICH, M.A. Biotecnologia 2011<\/em>. Rio de Janeiro: Edi\u00e7\u00f5es da Biblioteca Max Feffer do Instituto de Tecnologia ORT, 2012.<\/p>\n

MALARCZYK, D.; PANEK, J.; FRAC, M. Alternative molecular-based diagnostic methods of plant pathogenic fungi affecting berry crops\u2014a review. Molecules<\/em>, v. 24, n. 7, p. 1200, 2019.<\/p>\n

MARTINELLI, F.; SCALENGHE, R.; DAVINO, S.; PANNO, S.; SCUDERI, G.; RUISI, P.; VILLA, P.; STROPPIANA, D.; BOSCHETTI, M.; GOULART, L.R.; DAVIS, C.E.; DANDEKAR, A.M. Advanced methods of plant virus detection and disease management. Frontiers in Plant Science<\/em>, v. 11, p. 1092, 2020.<\/p>\n

MORGANTE, C.V.; BLAWID, R. An\u00e1lise da express\u00e3o g\u00eanica pela t\u00e9cnica de PCR quantitativa em tempo real: princ\u00edpios e fundamentos. Embrapa Semi\u00e1rido, Documentos<\/em>, n. 278, 2016. 63 p.<\/p>\n

NADEEM, M.A.; NAWAZ, M.A.; SHAHID, M.Q.; DO\u011eAN, Y.; COMERTPAY, G.; YILDIZ, M.; HATIPO\u011eLU, R.; AHMAD, F.; ALSALEH, A.; LABHANE, N.; \u00d6ZKAN, H.; CHUNG, G.; BALOCH, F.S. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology & Biotechnological Equipment<\/em>, v. 32, n. 2, p. 261-285, 2018.<\/p>\n

OLIVEIRA, E.M.M.; OLIVEIRA, T.C.; SOUZA, A.M.; SANTOS, T.F.; LIMA, I.S. Desenho de primers degenerados atrav\u00e9s de bioinform\u00e1tica. Embrapa Agroind\u00fastria de Alimentos, Comunicado T\u00e9cnico<\/em>, n. 208, 2015. 4 p.<\/p>\n

QUISEN, R.C.; \u00c2NGELO, P.C.S. Manual de procedimentos do Laborat\u00f3rio de Cultura de Tecidos da Embrapa Amaz\u00f4nia Ocidental. Embrapa Amaz\u00f4nia Ocidental, Documentos<\/em>, n. 61, 2008. 44 p.<\/p>\n

ROMANO, E.; BRASILEIRO, A.C.M. Extra\u00e7\u00e3o de DNA de plantas. Biotecnologia, Ci\u00eancia e Desenvolvimento<\/em>, v. 2, n. 9, p. 40-43, 1999.<\/p>\n

RUBIO, L.; GALIPIENSO, L.; FERRIOL, I. Detection of plant viruses and disease management: relevance of genetic diversity and evolution. Frontiers in Plant Science<\/em>, v. 11, p. 1092, 2020.<\/p>\n

SAMBROOK, J.; FRITSCH, E.F.; MANIATIS, T. Molecular Cloning: A Laboratory Manual<\/em>. 2. ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. 1659 p. .<\/p>\n

ZAHA, A.; FERREIRA, H.B.; PASSAGLIA, L.M.P. Biologia molecular b\u00e1sica. 5. ed. Porto Alegre: Artmed, 2014.<\/p>\n

\n","protected":false},"excerpt":{"rendered":"

N\u00edvel Mestrado\/Doutorado Natureza Concentrada Cr\u00e9ditos 04 Carga hor\u00e1ria 60 horas Ementa Estudos de t\u00e9cnicas moleculares utilizadas para detec\u00e7\u00e3o e identifica\u00e7\u00e3o de fitopat\u00f3genos. Informa\u00e7\u00f5es sobre extra\u00e7\u00e3o de \u00e1cidos nucleicos, PCR e suas varia\u00e7\u00f5es, qPCR, sequenciamento de Sanger, bioinform\u00e1tica b\u00e1sica para an\u00e1lise de sequ\u00eancias e elabora\u00e7\u00e3o de \u00e1rvores filogen\u00e9ticas. Estudos sobre t\u00e9cnicas sorol\u00f3gicas para identifica\u00e7\u00e3o de fitopat\u00f3genos. […]<\/p>\n","protected":false},"author":10266,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3280","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/pages\/3280","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/users\/10266"}],"replies":[{"embeddable":true,"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/comments?post=3280"}],"version-history":[{"count":4,"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/pages\/3280\/revisions"}],"predecessor-version":[{"id":6889,"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/pages\/3280\/revisions\/6889"}],"wp:attachment":[{"href":"https:\/\/agrarias.ufpr.br\/pgapv\/wp-json\/wp\/v2\/media?parent=3280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}